Selected Bibliography

Methane mitigation strategies

ARNDT, Claudia, et al. Full adoption of the most effective strategies to mitigate methane emissions by ruminants can help meet the 1.5 C target by 2030 but not 2050. Proceedings of the National Academy of Sciences, 2022, 119.20: e2111294119. https://doi.org/10.1073/pnas.211129411

BEAUCHEMIN, Karen A., et al. Invited review: Current enteric methane mitigation options. Journal of Dairy Science. 2022, vol. 105, n. 12, p. 9297-9326. https://doi.org/10.3168/jds.2022-22091

BEAUCHEMIN, Karen A., et al. The path to net-zero in dairy production: are pronounced decreases in enteric methane achievable?. Annual Review of Animal Biosciences, 2025, 13. https://doi.org/10.1146/annurev-animal-010324-113703

HRISTOV, Alexander N., et al. Feed additives for methane mitigation: Recommendations for testing enteric methane-mitigating feed additives in ruminant studies. Journal of Dairy Science, 2025, 108.1: 322-355. https://doi.org/10.3168/jds.2024-25050

WATERS, S. M., et al. The role of rumen microbiome in the development of methane mitigation strategies for ruminant livestock. Journal of Dairy Science, 2025. https://doi.org/10.3168/jds.2024-25778

Carbon stable isotopes in ruminants

CHANG, Jinfeng, et al. Revisiting enteric methane emissions from domestic ruminants and their δ13CCH4 source signature. Nature Communications, 2019, 10.1: 3420. https://doi.org/10.1038/s41467-019-11066-3

DE SMET, Stefaan, et al. Stable carbon isotope analysis of different tissues of beef animals in relation to their diet. Rapid Communications in mass spectrometry, 2004, 18.11: 1227-1232. https://doi.org/10.1002/rcm.1471

KLEVENHUSEN, Fenja, et al. Efficiency of monolaurin in mitigating ruminal methanogenesis and modifying C-isotope fractionation when incubating diets composed of either C3 or C4 plants in a rumen simulation technique (Rusitec) system. British Journal of Nutrition, 2009, 102.9: 1308-1317. https://doi.org/10.1017/S0007114509990262

KLEVENHUSEN, F., et al. The methanogenic potential and C-isotope fractionation of different diet types represented by either C3 or C4 plants as evaluated in vitro and in dairy cows. Australian Journal of Experimental Agriculture, 2008, 48.2: 119-123. https://doi.org/10.1071/EA07240

LOPES, J. C., et al. Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows. Journal of Dairy Science, 2016, 99.7: 5335-5344. https://doi.org/10.3168/jds.2015-10832

METGES, Cornelia; KEMPE, Klaus; SCHMIDT, Hanns-Ludwig. Dependence of the carbon-isotope contents of breath carbon dioxide, milk, serum and rumen fermentation products on the δ13C value of food in dairy cows. British Journal of Nutrition, 1990, 63.2: 187-196. https://doi.org/10.1079/BJN19900106

PASSEY, Benjamin H., et al. Carbon isotope fractionation between diet, breath CO2, and bioapatite in different mammals. Journal of Archaeological Science, 2005, 32.10: 1459-1470. https://doi.org/10.1016/j.jas.2005.03.015

PENNING, Holger, et al. Variation of carbon isotope fractionation in hydrogenotrophic methanogenic microbial cultures and environmental samples at different energy status. Global Change Biology, 2005, 11.12: 2103-2113. https://doi.org/10.1111/j.1365-2486.2005.01076.x

SCHULZE, E.; GIESE, W. Fractionation of carbon isotopes during carbohydrate fermentation in ruminants. Isotopes in Environmental and Health Studies, 1993, 29.1-2: 141-147. https://doi.org/10.1080/10256019308046147

SPONHEIMER, Matt, et al. An experimental study of carbon-isotope fractionation between diet, hair, and feces of mammalian herbivores. Canadian Journal of Zoology, 2003, 81.5: 871-876. https://doi.org/10.1139/z03-066

Continuous culture fermenter

BENNETT, Sarah L., et al. Effects of bacterial cultures, enzymes, and yeast-based feed additive combinations on ruminal fermentation in a dual-flow continuous culture system. Translational Animal Science, 2021, 5.2: txab026. https://doi.org/10.1093/tas/txab026

BRANDAO, Virginia LN; FACIOLA, Antonio P. Unveiling the relationships between diet composition and fermentation parameters response in dual-flow continuous culture system: a meta-analytical approach. Translational Animal Science, 2019, 3.3: 1064-1075. https://doi.org/10.1093/tas/txz019

CAGLIARI, Amanda Regina, et al. Evaluation of yeast-based additives on rumen fermentation in high-and low-concentrate diets using a dual-flow continuous culture system. Translational Animal Science, 2024, 8: txae169. https://doi.org/10.1093/tas/txae169

DILLARD, S. Leanne, et al. Evaluation of a single‐flow continuous culture fermenter system for determination of ruminal fermentation and enteric methane production. Journal of Animal Physiology and Animal Nutrition, 2019, 103.5: 1313-1324. https://doi.org/10.1111/jpn.13155

ISHLAK, A.; GÜNAL, M.; ABUGHAZALEH, A. A. The effects of cinnamaldehyde, monensin and quebracho condensed tannin on rumen fermentation, biohydrogenation and bacteria in continuous culture system. Animal Feed Science and Technology, 2015, 207: 31-40. https://doi.org/10.1016/j.anifeedsci.2015.05.023

MUETZEL, Stefan, et al. Evaluation of a stratified continuous rumen incubation system. Animal Feed Science and Technology, 2009, 151.1-2: 32-43. https://doi.org/10.1016/j.anifeedsci.2008.11.001

QUINN, Loyd Y. Continuous Culture of Ruminal Microorganisms in Chemically Defined Medium: I. Design of Continuous-Culture Apparatus. Applied Microbiology, 1962, 10.6: 580-582. https://doi.org/10.1128/am.10.6.580-582.1962

TEATHER, R. M.; SAUER, F. D. A naturally compartmented rumen simulation system for the continuous culture of rumen bacteria and protozoa. Journal of Dairy Science, 1988, 71.3: 666-673. https://doi.org/10.3168/jds.S0022-0302(88)79605-8

WENNER, B. A., et al. Dual-flow continuous culture fermentor system updated to decrease variance of estimates of digestibility of neutral detergent fiber. Applied Animal Science, 2021, 37.4: 445-450. https://doi.org/10.15232/aas.2021-02144

WENNER, B. A., et al. Effect of increasing dietary levels of a palmitic acid-enriched supplement on fiber digestibility, rumen fermentation, and microbial composition in high fiber diets. Journal of dairy science, 2025. https://doi.org/10.3168/jds.2024-25779

WENNER, B. A., et al. Evaluation of methane mitigation by organic feed additives in dual-flow continuous culture. JDS communications, 2024. https://doi.org/10.3168/jdsc.2024-0673